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KMID : 0043319970200050486
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Archives of Pharmacal Research
1997 Volume.20 No. 5 p.486 ~ p.490
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Resolution of Salbutamol Enantiomers in Human Urine by Reversed-phase High Performance Liquid Chromatography after Derivatization with (S)-(-)-α-methylbenzyl isocyanate
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Kim Kyeong-Ho
Kim Tae-Kyun
Kwon Young-Hee
Sohn Young-Teak
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Abstract
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A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with (S)-(-)--methyl-benzyl isocyanate, the diastereomeric derivatives were resolved on octadecylsilan column using 47% methanol as a mobile phase with fluorescence detection. The detection limit of each enantiomer was 10 ng/ml (S/N=3).
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KEYWORD
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Chiral separation, HPLC, Chiral derivatizing reagent, Salbutamol, Enantiomer, (S)-(-)-a-methylbenzyl isocyanate
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